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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

PCR

PCR

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Related Experiment Video

Updated: Mar 27, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

Handheld real-time PCR device.

Christian D Ahrberg¹, Bojan Robert Ilic², Andreas Manz¹

¹KIST-Europe, Microfluidics Group, Campus E7.1, 66111 Saarbrücken, Germany. pavel.neuzil@gmail.com.

|January 13, 2016

Summary

This summary is machine-generated.

Researchers developed a compact, real-time polymerase chain reaction (PCR) system for rapid infectious disease detection. This portable device can identify even a single DNA copy, enabling faster diagnostics in remote settings.

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Last Updated: Mar 27, 2026

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Published on: February 23, 2018

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Published on: May 25, 2015

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

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Published on: November 28, 2016

Area of Science:

Biotechnology
Molecular Biology
Medical Diagnostics

Background:

Current real-time polymerase chain reaction (PCR) systems can be bulky and complex.
There is a need for portable diagnostic tools for rapid detection of infectious agents.
Miniaturization of laboratory equipment is crucial for point-of-care applications.

Purpose of the Study:

To develop and characterize one of the smallest autonomous real-time PCR systems.
To assess the performance of the miniaturized PCR system for nucleic acid amplification.
To evaluate the potential of the device for rapid infectious disease diagnostics.

Main Methods:

Designed and constructed a compact real-time PCR system (100 mm × 60 mm × 33 mm).
Utilized virtual reaction chambers (VRCs) with approximately 200 nL sample volumes.
Performed 40-cycle amplification of an H7N9 gene amplicon and analyzed standard curves.

Main Results:

Achieved a standard curve slope of -3.02 ± 0.16 cycles/decade, indicating high amplification efficiency (0.91 ± 0.05 per cycle).
Demonstrated the system's capability to detect a single deoxyribonucleic acid (DNA) copy.
Validated the performance using a 40-cycle amplification of an H7N9 gene target.

Conclusions:

The developed handheld PCR device is highly sensitive and efficient.
Its small size and autonomous operation make it suitable for field use.
The technology holds significant potential for rapid, point-of-care infectious disease detection.