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Production of Formed Elements

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...

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Updated: Mar 27, 2026

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

Producing megakaryocytes from a human peripheral blood source.

Nikola Ivetic¹, Ishac Nazi², Nadia Karim²

¹Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario.

|January 13, 2016

Summary

This study shows how to grow mature megakaryocytes from human stem cells. This method allows for more cells for studying platelet disorders.

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Isolation of Mouse Megakaryocyte Progenitors

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Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation

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Isolation of Mouse Megakaryocyte Progenitors

Isolation of Mouse Megakaryocyte Progenitors

Published on: May 20, 2021

Area of Science:

Hematology
Stem Cell Biology
Cell Culture

Background:

Cultured megakaryocytes are valuable for studying human diseases.
Generating sufficient megakaryocytes for research is challenging.
This study focuses on developing mature megakaryocytes from peripheral blood hematopoietic stem and progenitor cells (HSPCs).

Purpose of the Study:

To describe and evaluate an expansion process for generating mature megakaryocytes from peripheral blood-derived HSPCs.
To assess the efficacy of thrombopoietin (TPO) in stimulating megakaryocyte growth, maturation, and morphology.

Main Methods:

Isolation of CD34+ HSPCs from peripheral blood via positive selection.
Expansion of HSPCs using an optimal CD34+ expansion supplement.
Culture of expanded cells with TPO and evaluation using flow cytometry and electron microscopy.

Main Results:

HSPCs expanded nine-fold after 4 days of culture.
TPO stimulation (20 ng/mL) for 8 days yielded a 2.9-fold increase in megakaryocytic cells.
83% of live cells expressed CD41a+ (megakaryocyte commitment) and 50% expressed CD42b+ (maturation).
Over 1.0 × 10^6 mature, polyploid megakaryocytes capable of proplatelet formation were generated.

Conclusions:

Peripheral blood HSPCs can be successfully expanded and differentiated into functional, mature megakaryocytes.
This process provides a sufficient number of cells for morphological studies.
The method supports research into platelet production disorders and factors affecting platelet production.