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Updated: Mar 27, 2026
12:12
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
Published on: February 8, 2016
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Surface-modified yeast cells: A novel eukaryotic carrier for oral application
Elisabeth E Kenngott1, Ruth Kiefer1, Nicole Schneider-Daum2
1Molecular and Cell Biology, Saarland University, D-66123 Saarbrücken, Germany.
Summary
Researchers developed novel yeast carriers that target specific gut cells for oral drug delivery. This bioadhesive system enhances the targeted delivery of antigens or drugs, improving oral administration efficacy.
Area of Science:
- Biotechnology
- Drug Delivery Systems
- Microbiology
Background:
- Effective targeting of M cells in Peyer's patches is crucial for oral delivery systems.
- Developing particulate carriers that bind to these cells is a key challenge.
Purpose of the Study:
- To establish a novel carrier system for targeted oral delivery.
- To utilize yeast Saccharomyces cerevisiae modified with invasin derivatives for enhanced M cell binding.
Main Methods:
- Engineered yeast Saccharomyces cerevisiae to express invasin derivatives on their cell surface.
- Tested binding affinity of modified yeast to human HEp-2 and M-like cells in vitro.
- Evaluated in vivo targeting of Peyer's patches using the most effective yeast derivative.
Main Results:
- Invasin derivatives were successfully expressed on the yeast cell surface.
- Recombinant yeast showed improved binding to HEp-2 and M-like cells.
- The integrin-binding domain of Yersinia enterocolitica invasin demonstrated the most effective targeting of Peyer's patches in vivo.
Conclusions:
- Cell surface-modified yeasts represent a novel bioadhesive eukaryotic carrier system.
- This system offers efficient and targeted oral delivery of antigens or drugs.
- The approach holds promise for advancing oral drug and antigen delivery technologies.
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