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Related Experiment Video

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Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Promiscuous tumor targeting phage proteins.

Amanda L Gross¹, James W Gillespie¹, Valery A Petrenko²

¹Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.

Protein Engineering, Design & Selection : PEDS

|January 15, 2016

Summary

This summary is machine-generated.

Researchers developed novel

Keywords:

lung cancer multi-target selection pancreatic cancer phage display targeted nanomedicines

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Area of Science:

Biotechnology and Nanomedicine
Oncology and Cancer Research

Background:

Targeted cancer therapies face challenges due to tumor heterogeneity.
Existing cancer cell-specific ligands struggle to address diverse cancer phenotypes.
A need exists for 'promiscuous' ligands targeting receptors across various cancer types.

Purpose of the Study:

To identify and develop novel 'promiscuous' targeting ligands for pan-cancer applications.
To evaluate the efficacy of these ligands in delivering chemotherapy to heterogeneous tumors.

Main Methods:

A novel selection scheme was employed to identify promiscuous phage fusion proteins with pan-cancer cell binding.
Phage fusion proteins were engineered and tested for specificity against lung and pancreatic cancer cells.
Doxorubicin-loaded liposomes were modified with selected phage fusion proteins (GSLEEVSTL, GEFDELMTM).

Main Results:

Promiscuous phage fusion proteins with conserved structural motifs were identified.
Modified liposomes demonstrated significantly enhanced cytotoxicity (up to 8.1-fold) in multiple cancer cell lines.
Targeted delivery via promiscuous ligands proved effective against heterogeneous tumor cell populations.

Conclusions:

Promiscuous phage proteins represent a promising class of targeting ligands for heterogeneous tumors.
This approach enhances the efficacy of nanomedicine delivery in cancer treatment.
The findings support the clinical translation of targeted therapies for diverse cancer types.