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Transient Ion-Pair Separations for Electrospray Mass Spectrometry.

Hanghui Liu1,2, Lily Lam1,2, Bert Chi1,2

  • 1Senomyx, Inc., 4767 Nexus Centre Drive, San Diego, California 92121, United States.

Analytical Chemistry
|January 15, 2016
PubMed
Summary
This summary is machine-generated.

A new transient ion-pair separation (TIPS) method for liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) avoids ion suppression and source contamination. This technique injects the ion-pairing reagent (IPR) onto the column, creating a temporary coating that elutes analytes before the IPR.

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Mass Spectrometry

Background:

  • Traditional ion-pair chromatography (IPC) for LC-ESI-MS suffers from ion suppression and ion source contamination due to the presence of ion-pairing reagents (IPRs) in the mobile phase.
  • These drawbacks limit the sensitivity and robustness of LC-ESI-MS analyses using IPC.

Purpose of the Study:

  • To introduce a novel transient ion-pair separation (TIPS) approach for LC-ESI-MS that eliminates the need for IPRs in the eluent.
  • To address the limitations of conventional IPC by preventing IPRs from entering the mass spectrometer.

Main Methods:

  • A high-retention factor IPR is injected onto the chromatography column, forming a transient, regional coating.
  • Analytes are subsequently injected and interact with this dynamic coating.
  • A specific eluent gradient is employed to elute analytes before the IPR, which is then washed from the column to waste.

Main Results:

  • Demonstrated successful separation of inorganic ions (iodate and nitrate) using TIPS on a reverse-phase column.
  • The TIPS method effectively prevents IPRs from reaching the mass spectrometer, thereby avoiding ion suppression and ion source contamination.
  • Reproducible separation was achieved due to the transient but well-defined regional coating.

Conclusions:

  • Transient ion-pair separation (TIPS) offers a viable alternative to conventional IPC for LC-ESI-MS.
  • This method significantly enhances analytical performance by mitigating ion suppression and contamination issues.
  • The generic nature of TIPS suggests potential applicability to other separation modes beyond LC-MS.