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Updated: Mar 27, 2026

Author Spotlight: A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods
Published on: April 28, 2023
Generalizability and Effectiveness of Butterfly Phlebotomy in Reducing Hemolysis
Douglas P Barnaby1, Andrew Wollowitz1, Deborah White1
1Department of Emergency Medicine of Albert Einstein College of Medicine, Bronx, NY.
Objectives:
The objective was to test the hypothesis that exclusive use of butterfly needles for phlebotomy, compared with sample collection via intravenous (IV) catheter, will reduce rates of sample hemolysis.
Methods:
This was an observational study of hemolysis rates before and after implementation of a "butterfly-only" phlebotomy protocol. Weekly hemolysis rates, generated by the central laboratory, were collected and the proportion of hemolyzed specimens before and after protocol implementation were compared.
Results:
Prior to institution of the butterfly-only protocol, 11.3% of specimens sent from the emergency department were reported as hemolyzed. During the postintervention period, only 4.3% of specimens were hemolyzed for a proportionate decrease of 62% and an absolute decrease of 7.0% (95% confidence interval = 6.6% to 7.3%).
Conclusions:
Use of a butterfly-only phlebotomy protocol cuts hemolysis rates by more than half when compared with IV catheter phlebotomy.

