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[HPLC-PDA-CAD Fingerprints of Salt Anemarrhenae Rhizoma]
Objective:
To establish the HPLC-PDA-CAD fingerprints and to determine its seven main constituents so as to provide a reliable evidence for the scientific evaluation and quality control of salt Anemarrhenae Rhizoma.
Methods:
The chromatographic fingerprint was obtained with Thermo Hypersil C18 column (250 mm x 4.6 mm, 5 μm) and gradient eluted with acetonitrile and water; The CAD parameters were pressure of 241. 3 kPa, filter of high and range of 200 pA. The detection wavelength of PDA was set at 258 nm.
Results:
The common mode of HPLC-PDA-CAD fingerprint of salt Anemarrhenae Rhizoma was set up. There were 5 PDA and 12 CAD common peaks in the fingerprints. Timosaponin B II, anemarsaponin E, timosaponin B III, timosaponin A III, neomangiferin, mangiferin and baohuoside I were identified in fingerprints and determined.
Conclusion:
The established HPLC-PDA-CAD fingerprint method is accurate, reliable, and has a good reproducibility and precision, which can be used for the quality control of salt Anemarrhenae Rhizoma.
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