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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Mar 27, 2026

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

Flow cytometry: basic principles and applications.

Aysun Adan¹, Günel Alizada², Yağmur Kiraz^1,2

¹a Faculty of Life and Natural Sciences , Abdullah Gül University , Kayseri , Turkey and.

Critical Reviews in Biotechnology

|January 16, 2016

Summary

Flow cytometry analyzes single cells using light scattering, enabling detailed analysis of complex cell populations. This review covers its principles, applications like immunophenotyping, and future prospects.

Keywords:

Apoptosis cytokines flow cytometer fluorescence fluorescent-activated cell sorting histogram immunophenotyping light scatter

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Area of Science:

Biotechnology
Cell Biology
Immunology

Background:

Flow cytometry is an advanced instrument for simultaneous measurement of single-cell physical characteristics.
It utilizes light scattering properties, often enhanced by dyes or antibodies targeting cell molecules.
This technology allows for rapid, detailed analysis of complex cellular populations.

Purpose of the Study:

To review the general principles of flow cytometry.
To discuss selected applications including immunophenotyping, apoptosis analysis, and cytokine detection.
To provide a foundational understanding of flow cytometry technology, data analysis, and interpretation.

Main Methods:

The review synthesizes information on flow cytometry principles and applications.
It covers techniques for analyzing peripheral blood cells, apoptosis, and cytokine detection.
Methods for data analysis and interpretation are also discussed.

Main Results:

Flow cytometry offers a powerful tool for rapid, detailed analysis of cellular characteristics.
Key applications include immunophenotyping, apoptosis assessment, and cytokine detection.
Recent advancements indicate a growing importance and expanding capabilities of this technology.

Conclusions:

Flow cytometry is essential for understanding cell biology and immunology.
The technology provides critical insights into cell populations and disease states.
Continued advancements promise to further enhance its diagnostic and research utility.