Related Experiment Video
Updated: Mar 27, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
Structure elucidation of uniformly (13)C labeled small molecule natural products
Mikhail Reibarkh1, Thomas P Wyche2, Josep Saurí1
1Process and Analytical Chemistry, NMR Structure Elucidation Group, Merck Research Laboratories, Rahway, NJ, United States.
Abstract:
Utilization of (2)H, (13)C, and (15)N isotopically labeled proteins and peptides is now routine in biomolecular NMR investigations. The widespread availability of inexpensive, uniformly (13) C enriched glucose now makes it possible to isolate uniformly (13)C labeled natural products from microbial fermentation. We now wish to describe an approach for the rapid structural characterization of uniformly (13)C labeled natural products that avoids the pitfalls of relying on parameters typically employed in biomolecular NMR studies.
Related Concept Videos
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Carbon-13 (¹³C) NMR: Overview
Mass Spectrum: Interpretation
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
¹H NMR Signal Integration: Overview

