Related Experiment Video

Updated: Mar 27, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

25.1K

Structure elucidation of uniformly 13C labeled small molecule natural products

Mikhail Reibarkh, Thomas P Wyche, Josep Saurí

    Magnetic Resonance in Chemistry : MRC
    |January 16, 2016
    PubMed

    More Related Videos

    Author Spotlight: Plant Primary Organs Profiling Using 13C6-Glucose Labeling and LC-MS04:32

    Author Spotlight: Plant Primary Organs Profiling Using 13C6-Glucose Labeling and LC-MS

    Published on: March 22, 2024

    1.5K
    Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy14:55

    Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

    Published on: September 17, 2017

    16.2K

    Related Concept Videos

    ¹³C NMR: ¹H–¹³C Decoupling01:04

    ¹³C NMR: ¹H–¹³C Decoupling

    The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
    A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
    2.1K
    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

    When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
    1.8K
    Carbon-13 (¹³C) NMR: Overview01:10

    Carbon-13 (¹³C) NMR: Overview

    Carbon-13 is a naturally occurring NMR-active isotope of carbon with a low natural abundance of 1.1%. In contrast, carbon-12 is the most abundant isotope of carbon with zero nuclear spin. Therefore, it is NMR inactive. The gyromagnetic ratio of carbon-13 is smaller than that of protons. As a result, carbon-13 resonance is about 6000 times weaker than proton resonance. For a given magnetic field strength, the resonance frequency of carbon-13 is about one-fourth of the resonance frequency for...
    9.3K
    Mass Spectrum: Interpretation01:24

    Mass Spectrum: Interpretation

    An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
    4.2K
    2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

    2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

    Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
    1.6K
    ¹H NMR Signal Integration: Overview00:58

    ¹H NMR Signal Integration: Overview

    The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
    4.1K