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Bioorthogonal Click Chemistry-Based Synthetic Cell Glue
Heebeom Koo1, Myunghwan Choi1,2, Eunha Kim3,4
1Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, 65 Landsdowne St., UP-5, Cambridge, MA, 02139, USA.
Small (Weinheim an Der Bergstrasse, Germany)
|January 16, 2016
Summary
Researchers developed a novel chemical cell glue using bioorthogonal click chemistry. This method enables stable in vivo cell-cell adhesion for potential therapeutic cell delivery and interaction studies.
Area of Science:
- Biomaterials Science
- Cell Biology
- Chemical Biology
Background:
- In vitro cell adhesion methods exist but lack in vivo applicability.
- Developing robust in vivo cell assembly techniques is crucial for therapeutic applications.
Purpose of the Study:
- To introduce a stable and robust chemical cell glue for in vivo applications.
- To demonstrate the viability and stability of chemically glued cells in physiological conditions.
Main Methods:
- Utilizing bioorthogonal click chemistry with tetrazine (Tz) and trans-cyclooctene (TCO) for cell surface conjugation.
- Assessing cell viability and attachment stability under shear stress in microfluidic channels.
- Tracking the in vivo behavior of injected, chemically glued Jurkat T cells in mice.
Main Results:
- Covalent cell-cell bonds formed within 10 minutes in aqueous conditions.
- Glued cell pairs exhibited stable attachment under high shear stress (20 dyn cm(-2)).
- Injected cell pairs demonstrated circulation and lung tissue infiltration in live mice.
Conclusions:
- The developed chemical cell glue shows high stability and robustness for in vivo applications.
- Chemically glued cell pairs are viable and can be tracked in vivo.
- This technology holds promise for therapeutic cell delivery and in vivo cell-cell interaction studies.
