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Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
Published on: March 28, 2014
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A quantitative cell modeling and wound-healing analysis based on the Electric Cell-substrate Impedance Sensing (ECIS)
Jen Ming Yang1, Szi-Wen Chen2, Jhe-Hao Yang3
1Department of Chemical and Materials Engineering, Chang Gung University, Taoyuan, Taiwan.
Computers in Biology and Medicine
|January 17, 2016
Summary
This study presents a quantitative model for keratinocyte cell growth and wound healing using Electric Cell-substrate Impedance Sensing (ECIS). The research found that negative pressure of 125mmHg accelerated the wound healing process in these cells.
Area of Science:
- Biophysics
- Cell Biology
- Biomedical Engineering
Background:
- Quantitative modeling of cell growth and wound healing is crucial for understanding tissue regeneration.
- Electric Cell-substrate Impedance Sensing (ECIS) offers a non-invasive method for monitoring cellular behavior.
- Previous work established a mathematical model for cell growth analysis using ECIS data.
Purpose of the Study:
- To assess the applicability of a previously developed mathematical model for keratinocyte cell growth analysis.
- To quantitatively evaluate the wound-healing process of keratinocyte cells under varying pressures using an electrical assay.
- To introduce and utilize novel indicators, cell power and cell electroactivity, for characterizing cell biophysical behavior.
Main Methods:
- Utilized Electric Cell-substrate Impedance Sensing (ECIS) for continuous monitoring of cell impedance.
- Applied a quantitative mathematical model to analyze time-series ECIS data for cell growth.
- Developed an electrical wound-healing assay and employed wavelet transform for multi-scale analysis of cell power and electroactivity.
Main Results:
- The mathematical model demonstrated a good fit for keratinocyte cell growth data obtained via ECIS.
- Quantitative analysis revealed that a negative pressure of 125mmHg significantly accelerated keratinocyte wound healing.
- Novel indicators, cell power and cell electroactivity, provided quantitative insights into cell biophysical responses.
Conclusions:
- The developed mathematical model is effective for keratinocyte cell growth modeling and analysis.
- Negative pressure, specifically 125mmHg, optimizes the wound healing rate in keratinocyte cell cultures.
- The study introduces valuable quantitative metrics for assessing cell biophysical behavior during wound repair.
