Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

5.1K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

1.7K
1.7K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

6.3K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
6.3K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.9K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.9K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

7.1K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
7.1K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

9.2K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PDIA1 promotes androgen receptor activation and prostate cancer cell survival through enhancing HMMR stability.

FEBS letters·2026
Same author

The current status and influencing factors of prophylactic antibacterial drug use in patients with hypertriglyceridemic acute pancreatitis.

Naunyn-Schmiedeberg's archives of pharmacology·2026
Same author

USP2 is an androgen-repressed survival factor that stabilises oncoproteins to facilitate therapy resistance in prostate cancer.

Cell death & disease·2026
Same author

Persistent Vomiting Among Children With Acute Gastroenteritis: A Secondary Analysis of a Randomized Clinical Trial.

JAMA network open·2026
Same author

Bacteremia and Bacterial Meningitis Among Low-Risk Febrile Infants Aged 29 to 60 Days.

JAMA pediatrics·2026
Same author

PRSS22 inhibits HMOX1-mediated ferroptosis and induces osteopontin cleavage to promote M2 macrophage polarization and colitis-associated carcinogenesis.

Oncogene·2026
Same journal

A Staufen1-mediated decay pathway influences the local transcriptome in axons.

Translation (Austin, Tex.)·2018
Same journal

Regulation of gene expression by translation factor eIF5A: Hypusine-modified eIF5A enhances nonsense-mediated mRNA decay in human cells.

Translation (Austin, Tex.)·2017
Same journal

Dissecting limiting factors of the Protein synthesis Using Recombinant Elements (PURE) system.

Translation (Austin, Tex.)·2017
Same journal

The utilization of selenocysteine-tRNA<sup>[Ser]Sec</sup> isoforms is regulated in part at the level of translation <i>in vitro</i>.

Translation (Austin, Tex.)·2017
Same journal

PRIMA: a gene-centered, RNA-to-protein method for mapping RNA-protein interactions.

Translation (Austin, Tex.)·2017
Same journal

Class II members of the poly(A) binding protein family exhibit distinct functions during Arabidopsis growth and development.

Translation (Austin, Tex.)·2017
See all related articles

Related Experiment Video

Updated: Mar 27, 2026

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

905

Signaling crosstalk between the mTOR complexes.

Jianling Xie1, Chris G Proud1

  • 1Centre for Biological Sciences; University of Southampton; Southampton, UK.

Translation (Austin, Tex.)
|January 19, 2016
PubMed
Summary
This summary is machine-generated.

The mechanistic target of rapamycin (mTOR) pathway involves two complexes, mTORC1 and mTORC2, that regulate cell growth. Recent findings reveal intricate crosstalk between these complexes, impacting human diseases and drug resistance.

Keywords:
SIN1TSC2mRNA translationmTORC1mTORC2protein synthesis

More Related Videos

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

14.9K
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

20.0K

Related Experiment Videos

Last Updated: Mar 27, 2026

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

905
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

14.9K
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

20.0K

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The mechanistic target of rapamycin (mTOR) is a key protein kinase regulating cellular anabolic processes like protein synthesis.
  • mTOR exists in two distinct multi-protein complexes, mTORC1 and mTORC2, each with unique targets and functions.
  • While sensitive to rapamycin differently, mTORC1 and mTORC2 share upstream regulators and exhibit linked activation patterns.

Purpose of the Study:

  • To summarize recently discovered features of crosstalk between mTORC1 and mTORC2.
  • To discuss the implications of aberrant mTOR complex crosstalk in human diseases.
  • To explore the role of mTOR complex crosstalk in the development of drug resistance.

Main Methods:

  • Literature review of recent studies on mTORC1 and mTORC2 signaling.
  • Analysis of shared upstream signaling molecules (e.g., PI3K, TSC).
  • Examination of feedback mechanisms influencing mTOR complex activation.

Main Results:

  • mTORC1 and mTORC2, despite functional differences, are tightly regulated and interconnected.
  • Shared signaling pathways and feedback loops demonstrate complex crosstalk.
  • Aberrant crosstalk contributes to disease pathogenesis and therapeutic resistance.

Conclusions:

  • Understanding mTORC1/mTORC2 crosstalk is crucial for deciphering cellular regulation.
  • Dysregulated mTOR signaling pathways offer potential therapeutic targets.
  • Further research into mTOR complex interactions may yield novel strategies for disease treatment and overcoming drug resistance.