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Related Experiment Video

Updated: Mar 27, 2026

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

RNA sequencing of the nephron transcriptome: a technical note.

¹Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA; Kidney Research Institute, Seoul National University, Seoul, Korea.

Kidney Research and Clinical Practice

|January 19, 2016

Summary

This study profiles the kidney nephron transcriptome using RNA sequencing and microdissection. The resulting gene expression data offers a detailed view of nephron function and is publicly available for research.

Keywords:

Microdissection Nephron RNA sequencing Transcriptome

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Area of Science:

Nephrology
Molecular Biology
Genomics

Background:

Understanding kidney function requires profiling the transcriptome of individual nephron segments.
RNA sequencing (RNA-seq) provides a sensitive and unbiased method for transcriptomic analysis.

Purpose of the Study:

To investigate the transcriptome of glomeruli and 14 renal tubule segments using a combination of RNA-seq and microdissection.
To create a comprehensive gene expression dataset for the rat nephron.

Main Methods:

Rat kidneys were processed using collagenase digestion and manual microdissection under a stereomicroscope.
Polyadenylated messenger RNAs (poly(A)'-mRNAs) were isolated, converted to complementary DNAs (cDNAs), and prepared into sequencing libraries.
Illumina sequencing was performed, and sequence data were mapped to the rat reference genome for gene expression analysis.

Main Results:

RNA-seq successfully profiled the transcriptome of glomeruli and 14 distinct renal tubule segments.
The generated gene expression data demonstrated high consistency with existing knowledge of nephron function.
The transcriptomic data are publicly accessible via a dedicated webpage for further exploration.

Conclusions:

The combination of RNA-seq and microdissection is a powerful approach for detailed nephron transcriptome analysis.
This study provides a valuable resource for understanding kidney physiology and pathophysiology.
The accessible dataset facilitates future research into the transcriptomic landscape of the nephron.