Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intravenous transplantation of mesenchymal stem cells improves cardiac performance after acute myocardial ischemia in female rats.

Transplant international : official journal of the European Society for Organ Transplantation·2006

Same author

[Effects of mechanical tensile stress on the expression of ICAM-1 mRNA in osteoblasts differentiated from rBMSCs].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition·2006

Same author

[Effects of osteoporosis on experimental tooth movement in aged rats].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition·2006

Same author

MCALIGN2: faster, accurate global pairwise alignment of non-coding DNA sequences based on explicit models of indel evolution.

BMC bioinformatics·2006

Same author

[Managements of masked mastoiditis].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2006

Same author

Neuronal SIRT1 activation as a novel mechanism underlying the prevention of Alzheimer disease amyloid neuropathology by calorie restriction.

The Journal of biological chemistry·2006

Same journal

Reliability of A Vibration-Based Elastography Protocol For Assessing Achilles Tendon Stiffness Across Multiple Joint Angles In Elite Athletes.

Journal of visualized experiments : JoVE·2026

Same journal

Associations of Inflammatory and Coagulation Biomarkers with Kidney Injury Across Chronic and Acute Clinical Settings.

Journal of visualized experiments : JoVE·2026

Same journal

Intelligent Recommender Systems for Chinese Super League Fan Consumption Behavior Prediction.

Journal of visualized experiments : JoVE·2026

Same journal

A Battery of Quantitative Binocular Vision Tests for Adults: Testing Protocols.

Journal of visualized experiments : JoVE·2026

Same journal

Efficacy Analysis of Paiteling in Treating Persistent High-Risk Human Papillomavirus after Cervical Cancer Surgery.

Journal of visualized experiments : JoVE·2026

Same journal

Clinical Efficacy of Tissue-Bone Homeostasis Manipulation on Soft Tissue Balance and Function in Knee Osteoarthritis.

Journal of visualized experiments : JoVE·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 27, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray.

Lisa S Ramirez¹, Jun Wang²

¹Department of Chemistry, University at Albany, State University of New York; Multiplex Biotechnology Laboratory, Cancer Research Center.

Journal of Visualized Experiments : Jove

|January 19, 2016

Summary

This summary is machine-generated.

This study presents a novel, high-density antibody microarray fabrication method using DNA flow-patterning. This technique offers enhanced sensitivity and miniaturization for single-cell protein analysis in various research and clinical applications.

More Related Videos

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Published on: February 7, 2017

Related Experiment Videos

Last Updated: Mar 27, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Published on: February 7, 2017

Area of Science:

Biotechnology
Molecular Biology
Bioengineering

Background:

Antibody microarrays are valuable tools facing competition from newer high-throughput technologies.
Existing microarray fabrication methods can be resource-intensive and lack miniaturization.

Purpose of the Study:

To develop an improved antibody microarray technology with novel fabrication and features.
To enable high-density, miniaturized protein analysis for applications like single-cell studies.

Main Methods:

Fabrication using perpendicularly oriented DNA flow-patterning.
Production and purification of DNA-antibody conjugates.
Assessment of microarray quality, uniformity, and sensitivity.

Main Results:

Achieved high-density antibody microarrays with comparable uniformity and sensitivity to conventional methods.
Eliminated the need for specialized array printers, making fabrication accessible.
Developed microarray units 10 times smaller, enabling single-cell protein analysis.

Conclusions:

The novel DNA flow-patterning approach renovates antibody microarray technology.
This method provides a cost-effective, accessible, and miniaturized platform for protein analysis.
The technology has broad potential in biomarker detection, cell signaling, and clinical diagnostics.