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Published on: February 3, 2021
A shelf-stable fluorogenic isothermal amplification assay for the detection of Burkholderia pseudomallei
Jilien Michelle Wong Tzeling1, Chan Yean Yean
1Department of Medical Microbiology & Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. jilienwong@hotmail.com yeancyn@yahoo.com.
Abstract:
A shelf-stable loop-mediated isothermal amplification (LAMP) reagent for Burkholderia pseudomallei detection is described. The coupling of LAMP reagents with the indirect colorimetric indicator and consequently its lyophilization enable the simple evaluation of results without the need for any advance laboratory instruments. The reagents were found to have a stable shelf life of at least 30 days with well-maintained sensitivity and specificity.
