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Molecular Characterization and Subtyping of Blastocystis Species in Irritable Bowel Syndrome Patients from North

Rojaleen Das1, Shehla Khalil1, B R Mirdha1

  • 1Department of Microbiology,All India Institute of Medical Sciences, New Delhi, India.

Plos One
|January 20, 2016
PubMed

Insights

Blastocystis species, particularly subtype ST3, is significantly associated with Irritable Bowel Syndrome (IBS). This study found Blastocystis more frequently in IBS patients than in healthy controls, highlighting its potential role in the condition.

Area of Science:

  • Medical Parasitology
  • Gastroenterology
  • Molecular Biology

Background:

  • Blastocystis species are increasingly recognized for their potential role in human gastrointestinal disorders.
  • Understanding the association between Blastocystis subtypes and Irritable Bowel Syndrome (IBS) is crucial for diagnosis and treatment.

Purpose of the Study:

  • To identify Blastocystis species and its subtypes in patients with Irritable Bowel Syndrome (IBS).
  • To examine the association between Blastocystis colonization and IBS.
  • To investigate the prevalence of different Blastocystis subtypes in IBS patients and healthy controls.

Main Methods:

  • Detection of Blastocystis species using microscopy, in-vitro culture, and Polymerase Chain Reaction (PCR).
  • Subtyping of Blastocystis isolates using Sequence Tagged Site (STS) and sequencing of the SSU rRNA gene.
  • Comparison of Blastocystis prevalence between a cohort of 150 IBS patients and 100 healthy controls.

Main Results:

  • Blastocystis species were identified in 50 IBS patients and 15 healthy controls.
  • Subtype ST3 was the most prevalent (91%), followed by ST1 (9%).
  • A statistically significant association was found between Blastocystis colonization and IBS, with higher occurrence in IBS patients compared to controls.

Conclusions:

  • Blastocystis species, particularly subtype ST3, are significantly more prevalent in IBS patients than in healthy individuals.
  • While Blastocystis colonization is associated with IBS, no specific subtype was linked to particular clinical presentations of IBS.
  • The genetic diversity of Blastocystis species, as evidenced by SSU-rRNA gene polymorphism, warrants further investigation into their pathogenicity.

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