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Updated: Aug 11, 2026

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In vivo Dual Substrate Bioluminescent Imaging
Published on: October 11, 2011
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Design and Synthesis of an Alkynyl Luciferin Analogue for Bioluminescence Imaging
Rachel C Steinhardt1, Jessica M O'Neill1, Colin M Rathbun1
1Department of Chemistry, University of California, Irvine, Irvine, CA, USA.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 20, 2016
Abstract:
Herein, the synthesis and characterization of an alkyne-modified luciferin is reported. This bioluminescent probe was accessed using C-H activation methodology and was found to be stable in solution and capable of light production with firefly luciferase. The luciferin analogue was also cell permeant and emitted more redshifted light than d-luciferin, the native luciferase substrate. Based on these features, the alkynyl luciferin will be useful for a variety of imaging applications.

