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Updated: Mar 26, 2026
Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons
Published on: July 14, 2021
Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle
Jacqueline A Tickle1, Stuart I Jenkins1, Boris Polyak2
1Institute for Science & Technology in Medicine, School of Medicine, David Weatherall Building, Keele University, Staffordshire, ST5 5BG, UK.
Aim:
To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles.
Materials & Methods:
MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety.
Results:
Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'.
Conclusion:
These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.
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