Related Experiment Video
Updated: Mar 26, 2026
Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
Published on: February 27, 2026
Identification of novel noncoding transcripts in telomerase-negative yeast using RNA-seq.
Rachel O Niederer1, Nickolas Papadopoulos2, David C Zappulla1
1Department of Biology, Johns Hopkins University, Baltimore, MD, 21218 USA.
Researchers identified 112 novel long noncoding RNAs (lncRNAs) in yeast, with 41 specifically expressed in telomerase-negative cells. This discovery offers new insights into cellular senescence and adaptation mechanisms.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Telomerase maintains chromosome ends, and its loss causes telomere shortening and senescence.
- Telomere shortening is linked to aging in humans and cancer development.
- The role of noncoding RNAs (ncRNAs) in short-telomere-induced senescence is unclear.
Purpose of the Study:
- To investigate the involvement of ncRNAs in short-telomere-induced senescence in yeast.
- To identify novel long noncoding RNAs (lncRNAs) in Saccharomyces cerevisiae.
Main Methods:
- Bioinformatic analysis to identify putative novel lncRNAs.
- Expression analysis of lncRNAs in telomerase-negative yeast.
- Correlation analysis between lncRNA expression and adjacent gene expression.
Main Results:
- Identified 112 putative novel lncRNAs in Saccharomyces cerevisiae.
- Found 41 lncRNAs exclusively expressed in telomerase-negative yeast.
- Observed expression correlation between approximately half of the lncRNAs and their neighboring genes.
Conclusions:
- Novel lncRNAs are associated with short-telomere-induced senescence in yeast.
- Some lncRNAs may regulate transcription of adjacent genes.
- These findings suggest a new mechanism for adaptive changes in senescing yeast cells.
More Related Videos
09:21Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
Published on: October 22, 2018
11:24Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes
Published on: December 5, 2025
Related Concept Videos
03:21RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
02:24Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...