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Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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Simple and rapid quantification of phospholipids for supramolecular membrane transport assays
Robert Hein1, Can B Uzundal, Andreas Hennig
1Department of Life Sciences and Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany. a.hennig@jacobs-university.de.
Organic & Biomolecular Chemistry
|January 22, 2016
Abstract:
We introduce a simple (1)H NMR method for quantification of the phospholipid content of liposomes. The method is validated by comparison with the established Stewart assay, which revealed significant uncertainties in phospholipid quantification of established liposome preparations used in supramolecular membrane transport assays.
