Related Experiment Video
Updated: Mar 26, 2026
Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
Published on: January 26, 2015
Cell Surface-Anchored Fluorescent Probe Capable of Real-Time Imaging of Single Mast Cell Degranulation Based on
Yuji Oshikawa1, Kazuyuki Furuta2, Satoshi Tanaka2
1Graduate School of Pharmaceutical Sciences, Kyushu University , 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Abstract:
Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.
