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Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

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Local Extravascular Growth Factor Delivery in Myocardial Ischemia.

J J Lopez¹, M Simons¹

¹a Angiogenesis Research Center, Vascular Biology Unit, Cardiology Division, Department of Medicine, Harvard Medical School, and Beth Israel Hospital, Boston, Massachusetts, USA.

|January 22, 2016

Summary

Local delivery of angiogenic growth factors, basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), significantly enhanced coronary collateral circulation and myocardial blood flow in a porcine model of chronic ischemia.

Keywords:

Angiogenesis Collateral Circulation Growth Factors

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Area of Science:

Cardiovascular Research
Regenerative Medicine
Angiogenesis Studies

Background:

Ischemic myocardium often suffers from reduced blood supply.
Angiogenic growth factors like bFGF and VEGF show potential for stimulating new blood vessel growth.
Developing effective delivery methods for these factors is crucial for therapeutic applications.

Purpose of the Study:

To investigate the efficacy of exogenous growth factor delivery in promoting coronary collateral circulation.
To evaluate the role of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) in ischemic myocardium.
To assess the impact of local perivascular drug delivery on angiogenesis.

Main Methods:

Utilized a porcine ameroid constrictor model to induce chronic myocardial ischemia.
Administered bFGF via heparin alginate microspheres and VEGF via an implantable osmotic pump.
Quantified microvessel density using anti-von Willebrand antibody staining.
Assessed myocardial blood flow and perfusion using established physiological measurements.

Main Results:

Growth factor-treated pigs exhibited a significant increase in myocardial microvessel density compared to controls.
Resting collateral flow values were significantly higher in bFGF and VEGF treated animals.
Both bFGF and VEGF improved myocardial perfusion, preventing stress-induced performance decline.

Conclusions:

Local perivascular delivery of bFGF and VEGF effectively induces angiogenesis in chronically ischemic myocardium.
Exogenous growth factor administration can significantly improve coronary collateral circulation and myocardial blood flow.
This study supports the potential of targeted drug delivery for treating ischemic heart conditions.