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Updated: Mar 26, 2026
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Published on: November 27, 2016
Quantitative analysis of serotonin secreted by human embryonic stem cells-derived serotonergic neurons via
Xuefei Zhong1, Ling Hao1, Jianfeng Lu2
1School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
Abstract:
A CE-ESI-MRM-based assay was developed for targeted analysis of serotonin released by human embryonic stem cells-derived serotonergic neurons in a chemically defined environment. A discontinuous electrolyte system was optimized for pH-mediated online stacking of serotonin. Combining with a liquid-liquid extraction procedure, LOD of serotonin in the Krebs'-Ringer's solution by CE-ESI-MS/MS on a 3D ion trap MS was0.15 ng/mL. The quantitative results confirmed the serotonergic identity of the in vitro developed neurons and the capacity of these neurons to release serotonin in response to stimulus.
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