Related Experiment Video
Updated: Mar 26, 2026
Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
Published on: October 6, 2022
A NEW SPECTROPHOTOMETRIC METHOD FOR THE ASSAY OF LISINOPRIL
Aim:
A new spectrophotometric method for the assay of lisinopril using 2,4-dinitrophenol as reagent is described.
Material And Methods:
The method involved the addition of 2,4-dinitrophenol to lisinopril in methanol followed by absorbance measurement at 400 nm. Experimental conditions that provide wide linear range, maximum sensitivity, selectivity, accuracy and precisions were optimized.
Results And Discussions:
Beer's law was obeyed in the concentration range 2.0-14.0 μg/mL. Linear regression equation of calibration graph was A = 0.005 + 0.045 x concentration, with a regression coefficient (r) of 0.9995 (n = 8). The limits of detection (LOD) and quantification (LOQ) calculated according to the ICH guidelines were 0.42 and 1.42 μg/mL, respectively. Accuracy and precision of the assays were determined by computing the intra-day and inter-day variations at three different lisinopril concentrations; the intra-day and inter-day RSD was < 1.43% and accuracy was better than 1.72%.
Conclusions:
The proposed method is simple, easy to perform, sensitive, linear, precise, accurate and robust.
