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Updated: Mar 26, 2026

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System
Published on: May 5, 2020
Fast axonal transport in isolated axoplasm from the squid giant axon
Yuyu Song1, Minsu Kang2, Gerardo Morfini2
1Marine Biological Laboratory, Woods Hole, MA, USA; Yale School of Medicine, Department of Genetics and Howard Hughes Medical Institute, Boyer Center, New Haven, CT, USA.
Abstract:
The giant axon of the squid provides a unique cell biological model for analyzing the biochemistry and cell biology of the axon. These axons may exceed 500 μm in diameter and can be readily dissected. Once the surrounding small axons and connective tissue are removed, the axoplasm can be extruded as an intact cylinder of isolated cytoplasm. This isolated axoplasm is morphologically indistinguishable from the intact axon, but without permeability barriers. Fast axonal transport will continue for more than 4 h after extrusion and can be visualized in real time. By perfusing defined concentrations of proteins and/or reagents into the axoplasm, this preparation represents a powerful model for study of intracellular trafficking and its underlying molecular mechanisms.
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