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Published on: May 31, 2024
Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles
Guangsheng Wu1, Chao Feng2, Guangyan Hui3
1State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China; College of Marine Life Science, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong Province, China; Qingdao First Sanatorium, Jinan Military Region, No. 27 West Hong Kong Road, Qingdao 266071, Shandong Province, China.
Abstract:
MicroRNAs (miRNAs) play important roles in the osteogenic differentiation of stem cells. However, the application of miRNA in bone regeneration has been limited by its poor stability, low cellular uptake, and undesired immune response. In this study, chitosan (CS)/tripolyphosphate (TPP)/Hyaluronic Acid (HA) nanoparticles (CTH NPs) were prepared to deliver antimiR-138 to bone marrow mesenchymal stem cells (MSCs). The particle size, polydispersity index, and zeta potential of CTH NPs were related to the weight ratio of CS:TPP:HA. At optimum N/P ratio (20:1), the highest encapsulation efficiency was obtained. Both blank CTH NPs and CTH/antmiR-138 NPs exhibited no cytotoxicity to MSCs. A high transfection efficiency (nearly 70%) and significant enhancement of the osteogenesis of MSCs were observed. Above results demonstrated that CTH NPs was a potential candidate as an efficient non-viral miRNA vector to regulate the osteogenic differentiation of MSCs.
