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hsa_circ_0000285 facilitates thyroid cancer progression by regulating miR-127-5p/CDH2.

Bowei Zhang1,2, Qiaoling Li3, Zhe Song1,2

  • 1Department of Vascular and Thyroid Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Journal of Clinical Laboratory Analysis
|April 21, 2022
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Summary

Circular RNA hsa_circ_0000285 promotes thyroid cancer (THCA) by acting as a competing endogenous RNA. Downregulating circ_0000285 inhibits THCA cell proliferation and migration, offering a potential therapeutic target.

Keywords:
CDH2circRNAhsa_circ_0000285miR-127-5pthyroid cancer

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Thyroid cancer (THCA) is a significant endocrine malignancy, particularly in females.
  • Circular RNAs (circRNAs) are increasingly recognized for their regulatory roles in cancer development, including THCA.

Purpose of the Study:

  • To investigate the role and mechanism of hsa_circ_0000285 (circ_0000285) in thyroid cancer progression.
  • To explore circ_0000285's potential as a therapeutic target for THCA.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for expression profiling.
  • Cellular assays (CCK-8, wound healing) and Western blot to assess functional impacts.
  • In vivo xenograft models for tumor growth evaluation.
  • Bioinformatic analysis, RNA immunoprecipitation (RIP), and dual-luciferase reporter assays to elucidate molecular mechanisms.

Main Results:

  • hsa_circ_0000285 expression was significantly upregulated in THCA tissues and cells compared to normal controls.
  • Downregulation of circ_0000285 suppressed THCA cell proliferation and migration, induced apoptosis, and inhibited tumor growth in vivo.
  • circ_0000285 acts as a competing endogenous RNA (ceRNA) for miR-127-5p, leading to increased CDH2 expression and promoting THCA malignancy.

Conclusions:

  • hsa_circ_0000285 plays a crucial oncogenic role in thyroid cancer development.
  • The circ_0000285/miR-127-5p/CDH2 axis represents a novel regulatory pathway in THCA.
  • circ_0000285 inhibition presents a promising therapeutic strategy for thyroid cancer.