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Updated: Mar 26, 2026
High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Ultrasound-assisted extraction of hemicelluloses from grape pomace using response surface methodology
R Minjares-Fuentes1, A Femenia2, M C Garau2
1Área de Ingeniería Química, Departamento de Química, Universitat de les Illes Balears, Ctra Valldemossa Km 7.5, 07122 Palma de Mallorca, Spain; Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango., Av. Artículo 123 S/N, Fracc. Filadelfia, 35010 Gómez Palacio, DGO, Mexico.
Ultrasound-assisted extraction efficiently recovers hemicelluloses, including xyloglucans, mannans, and xylans, from grape pomace. This method shows promise for industrial-scale polysaccharide extraction at mild temperatures.
Area of Science:
- Biomass Valorization
- Green Chemistry
- Polysaccharide Extraction
Background:
- Grape pomace is a rich source of valuable polysaccharides like hemicelluloses.
- Efficient extraction methods are needed to valorize this agro-industrial byproduct.
- Ultrasound-assisted extraction (UAE) offers a promising approach for biomass processing.
Purpose of the Study:
- To optimize ultrasound-assisted extraction (UAE) of hemicelluloses from grape pomace.
- To maximize the yield of total hemicelluloses and specific polymers: xyloglucans (XLG), mannans (MAN), and xylans (XN).
- To determine optimal extraction parameters including time, solid:liquid ratio, and KOH concentration.
Main Methods:
- Ultrasound-assisted extraction (UAE) was performed at a mild temperature (20°C).
- A Central Composite Design (CCD) was employed for process optimization.
- Key variables investigated were extraction time, solid:liquid ratio, and KOH concentration.
Main Results:
- Optimized conditions yielded maximum hemicelluloses (∼7.9%), XLG (∼3.6%), MAN (∼1.1%), and XN (∼1.2%).
- Specific optimal parameters were determined for maximizing each target component.
- High agreement between experimental and predicted values confirmed model validity.
Conclusions:
- UAE is an effective technique for extracting hemicellulosic polysaccharides from grape pomace.
- The optimized process demonstrates potential for industrial-scale application.
- This study contributes to the sustainable valorization of grape processing waste.
