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Published on: December 2, 2022
Antimicrobial and antitumor activities of chitosan from shiitake stipes, compared to commercial chitosan from crab
Rao-Chi Chien1, Ming-Tsung Yen2, Jeng-Leun Mau1
1Department of Food Science and Biotechnology, National Chung Hsing University (NCHU), Taichung, Taiwan, ROC; NCHU/University of California at Davis, Plant and Food Biotechnology Center, NCHU, Taichung, Taiwan, ROC; Agricultural Biotechnology Center, NCHU, Taiwan, ROC.
Abstract:
Chitosan was prepared by alkaline N-deacetylation of chitin obtained from shiitake stipes and crab shells and its antimicrobial and antitumor activities were studied. Chitosan from shiitake stipes and crab shells exhibited excellent antimicrobial activities against eight species of Gram positive and negative pathogenic bacteria with inhibition zones of 11.4-26.8mm at 0.5mg/ml. Among chitosan samples, shiitake chitosan C120 was the most effective with inhibition zones of 16.4-26.8mm at 0.5mg/ml. In addition, shiitake and crab chitosan showed a moderate anti-proliferative effect on IMR 32 and Hep G2 cells. At 5mg/ml, the viability of IMR 32 cells incubated with chitosan was 68.8-85.0% whereas that of Hep G2 cells with chitosan was 60.4-82.9%. Overall, shiitake chitosan showed slightly better antimicrobial and antitumor activities than crab chitosan. Based on the results obtained, shiitake and crab chitosan were strong antimicrobial agents and moderate antitumor agents.
