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Naringenin-Mediated ATF3 Expression Contributes to Apoptosis in Human Colon Cancer

Hun Min Song1, Gwang Hun Park1, Hyun Ji Eo1

  • 1Department of Bioresource Sciences, Andong National University, Andong 760749, Republic of Korea.

Biomolecules & Therapeutics
|January 23, 2016
PubMed

Insights

Naringenin (NAR), a grapefruit flavonoid, induces apoptosis in human colon cancer cells by activating the transcription factor 3 (ATF3) via the p38 pathway. This mechanism enhances cancer cell death.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Naringenin (NAR), a flavonoid found in grapefruit, shows potential anti-cancer properties.
  • Activating transcription factor 3 (ATF3) plays a role in apoptosis in human colon cancer cells.

Purpose of the Study:

  • To investigate the molecular mechanism of NAR-induced ATF3 expression and apoptosis in human colon cancer cells.
  • To elucidate the role of the p38 pathway in NAR's anti-cancer effects.

Main Methods:

  • Cell viability assays and apoptosis detection.
  • Western blotting and qRT-PCR to assess protein and mRNA levels of ATF3.
  • Luciferase reporter assays to analyze ATF3 promoter activity.
  • Pharmacological inhibition of the p38 pathway.

Main Results:

  • NAR reduced colon cancer cell viability and induced apoptosis.
  • NAR dose-dependently increased ATF3 expression and promoter activity.
  • ATF3 overexpression enhanced NAR-mediated PARP cleavage, while knockdown attenuated it.
  • p38 pathway inhibition blocked NAR-induced ATF3 expression, promoter activation, and apoptosis.

Conclusions:

  • Naringenin induces apoptosis in human colon cancer cells.
  • The mechanism involves the activation of ATF3 expression via the p38 signaling pathway.
  • NAR represents a potential therapeutic agent for colon cancer.
Keywords:
Activating transcription factor 3Cancer chemopreventionHuman colon cancerNaringenin

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