Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

The linac coherent light source single particle imaging road map.

A Aquila, A Barty¹, C Bostedt²

¹Center for Free-Electron Laser Science, DESY , Notkestr. 85, 22607 Hamburg, Germany.

Structural Dynamics (Melville, N.Y.)

|January 23, 2016

Summary

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Other science opportunities at the FCC-ee.

European physical journal plus·2026

Same author

Strategy for pacemaker implantation following transcatheter tricuspid valve replacement: A real world single centre experience.

Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing·2025

Same author

Passive versus active educational interventions for nevus and melanoma classification: A randomized controlled study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2025

Same author

Eccentric hypertrophy impairs outcome after TAVR.

Clinical research in cardiology : official journal of the German Cardiac Society·2024

Same author

Correction: <i>Lactiplantibacillus plantarum</i> HEAL9 attenuates cognitive impairment and progression of Alzheimer's disease and related bowel symptoms in SAMP8 mice by modulating microbiota-gut-inflammasome-brain axis.

Food & function·2024

Same author

Unidirectional ray polaritons in twisted asymmetric stacks.

Nature communications·2024

This summary is machine-generated.

Intense X-ray imaging captures single particles in one shot using free-electron laser sources. Researchers are developing a roadmap for achieving atomic resolution in 3D imaging at these advanced light sources.

Area of Science:

Physics
Materials Science
Biophysics

Background:

Free-electron laser (FEL) sources provide intense femtosecond X-ray pulses for single-shot imaging.
Current coherent diffractive imaging (CDI) achieves resolutions of tens of nanometers for various samples.
Atomic resolution imaging remains a significant challenge in FEL-based CDI.

Purpose of the Study:

To summarize findings from the March 2014 LCLS workshop on atomic resolution imaging.
To outline the scientific and technical roadmap towards achieving atomic resolution 3D imaging.
To identify challenges and opportunities in single-shot CDI at FELs.

Main Methods:

Coherent diffractive imaging (CDI) using intense femtosecond X-ray pulses.
Analysis of experimental data from Linac Coherent Light Source (LCLS).

Related Experiment Videos

Discussion and synthesis of expert opinions from a dedicated workshop.

Main Results:

Progress in single-shot imaging of biological specimens, aerosols, and quantum systems.
Identification of key challenges in achieving atomic resolution, including data analysis and detector technology.
A roadmap outlining necessary advancements for future atomic resolution 3D imaging.

Conclusions:

Atomic resolution 3D imaging using single-shot CDI at FELs is an achievable goal.
Continued development of X-ray sources, optics, and detectors is crucial.
Interdisciplinary collaboration is essential to overcome current limitations and advance the field.