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Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo
Published on: July 1, 2013
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Cell-laden hydrogel/titanium microhybrids: Site-specific cell delivery to metallic implants for improved integration.
Geraldine Koenig1, Hayriye Ozcelik1, Lisa Haesler2
1Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, 11 Rue Humann, 67000 Strasbourg, France; Faculté de Chirurgie Dentaire, Université de Strasbourg, 8 rue Sainte Elisabeth, 67000 Strasbourg, France.
Acta Biomaterialia
|January 24, 2016
Summary
This study developed titanium/hydrogel microhybrids for improved porous titanium implant integration. These models facilitate testing cell behavior and implant modifications for better tissue integration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Porous titanium implants enhance integration in dental, orthopedic, and ENT fields.
- Cell-laden hydrogels offer improved host tissue integration by localizing cells and growth factors.
- Fast-gelling hydrogels are ideal for in situ applications, preventing cell dispersion.
Purpose of the Study:
- To design and validate a model system of cell-laden hydrogel-containing porous titanium implants.
- To investigate the effects of titanium presence on hydrogel properties and encapsulated cell behavior.
- To enable site-specific modification of titanium implants for heterogeneous tissue integration.
Main Methods:
- Fabrication of titanium microbead/hydrogel microhybrids using maleimide-dextran or maleimide-PVA based hydrogels.
- Co-culture of fibroblasts and vascular endothelial cells within the microhybrid system.
- Quantification of cell behavior, proliferation, and matrix metalloproteinase (MMP)-sensitive hydrogel effects using microscopy and qRT-PCR.
Main Results:
- Titanium presence minimally altered gel properties without affecting cell viability or gel formation.
- Encapsulated cells showed a preference for migrating towards titanium beads, with enhanced fibroblast proliferation.
- MMP-sensitive hydrogels promoted cell sprouting, and 3D structures achieved localized cellular distribution.
Conclusions:
- Titanium/hydrogel microhybrids serve as effective models for studying tissue-implant interactions and optimizing metallic implant modifications.
- Site-specific modification of titanium implants with cell-laden microgels is feasible.
- Microbead-based models facilitate characterization of titanium surface modifications using conventional analysis techniques.
