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Updated: Mar 26, 2026

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
Detection of Different DNA Animal Species in Commercial Candy Products
Marta Muñoz-Colmenero1, Jose Luis Martínez2, Agustín Roca1
1Laboratory of Genetics of Natural Resources, Area of Genetics, Dept. of Functional Biology, Univ. of Oviedo, Asturias, Spain.
Abstract:
Candy products are consumed all across the world, but there is not much information about their composition. In this study we have used a DNA-based approach for determining the animal species occurring in 40 commercial candies of different types. We extracted DNA and performed PCR amplification, cloning and sequencing for obtaining species-informative DNA sequences. Eight species were identified including fish (hake and anchovy) in 22% of the products analyzed. Bovine and porcine were the most abundant appearing in 27 samples each one. Most products contained a mixture of species. Marshmallows (7), jelly-types, and gummies (20) contained a significantly higher number of species than hard candies (9). We demonstrated the presence of DNA animal species in candy product which allow consumers to make choices and prevent allergic reaction.

