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Electrospun Nanofibrous Sheets for Selective Cell Capturing in Continuous Flow in Microchannels.

Young Ju Son1, Jihyun Kang1, Hye Sung Kim1

  • 1Department of Medical Biomaterials Engineering and §Institute of Bioscience and Biotechnology, Kangwon National University , Chuncheon 200-701, Republic of Korea.

Biomacromolecules
|January 27, 2016
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Summary

Researchers developed modified nanofibrous meshes for selectively capturing specific cells in microfluidic devices. These surface-modified materials demonstrate efficient and precise cell isolation from continuous flow systems.

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Area of Science:

  • Biomaterials Engineering
  • Microfluidics
  • Cell Separation

Background:

  • Selective cell capture is crucial for diagnostics and research.
  • Existing methods often lack efficiency or specificity in continuous flow systems.
  • Nanofibrous materials offer high surface area for modification.

Purpose of the Study:

  • To engineer electrospun nanofibrous meshes for selective cell capture.
  • To functionalize surfaces for specific cell binding in microfluidic channels.
  • To demonstrate efficient and selective isolation of biotinylated cells.

Main Methods:

  • Electrospinning of poly(ε-carprolactone) (PCL) and amine-functionalized block copolymers (PCL-PEI).
  • Surface modification via chemical tethering of biotinylated polyethylene glycol (PEG) to amine groups.
  • Quantitative fluorescence assay to determine biotinylation levels.
  • Microfluidic channel experiments to assess cell capture efficiency and selectivity.

Main Results:

  • Successfully created nanofibrous mats with tunable surface biotinylation.
  • Demonstrated increased avidin incorporation with higher biotinylated PEG ratios.
  • Achieved efficient and selective capture of biotinylated cells from a continuous medium flow.
  • Confirmed specific binding of biotinylated cells to avidin-coated mats.

Conclusions:

  • Surface-modified electrospun nanofibrous meshes enable selective cell capture.
  • The developed method allows for controlled surface functionalization for specific binding.
  • This technology shows promise for cell isolation applications in microfluidics.