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Published on: September 20, 2016
SUSD2 is frequently downregulated and functions as a tumor suppressor in RCC and lung cancer
Yingying Cheng1, Xiaolin Wang1, Pingzhang Wang1
1Peking University Center for Human Disease Genomics, Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
Abstract:
Sushi domain containing 2 (SUSD2) is type I membrane protein containing domains inherent to adhesion molecules. There have been few reported studies on SUSD2, and they have mainly focused on breast cancer, colon cancer, and HeLa cells. However, the expression and function of SUSD2 in other cancers remain unclear. In the present study, we conducted an integrated bioinformatics analysis based on the array data from the GEO database and found a significant downregulation of SUSD2 in renal cell carcinoma (RCC) and lung cancer. Western blotting and quantitative RT-PCR (qRT-PCR) confirmed that SUSD2 was frequently decreased in RCC and lung cancer tissues compared with the corresponding levels in normal adjacent tissues. The restoration of SUSD2 expression inhibited the proliferation and clonogenicity of RCC and lung cancer cells, whereas the knockdown of SUSD2 promoted A549 cell growth. Our findings suggested that SUSD2 functions as a tumor suppressor gene (TSG) in RCC and lung cancer.
Insights
Sushi domain containing 2 (SUSD2) acts as a tumor suppressor gene in renal cell carcinoma (RCC) and lung cancer. This study found SUSD2 is downregulated in these cancers, inhibiting their growth when restored.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Sushi domain containing 2 (SUSD2) is a type I membrane protein with adhesion molecule domains.
- Previous SUSD2 research primarily focused on breast cancer, colon cancer, and HeLa cells, leaving its role in other cancers largely unknown.
Purpose of the Study:
- To investigate the expression and function of SUSD2 in renal cell carcinoma (RCC) and lung cancer.
- To determine if SUSD2 acts as a tumor suppressor gene in these specific cancer types.
Main Methods:
- Integrated bioinformatics analysis using GEO database array data.
- Western blotting and quantitative RT-PCR (qRT-PCR) for SUSD2 expression validation.
- Functional assays involving SUSD2 restoration and knockdown in cancer cell lines.
Main Results:
- Significant downregulation of SUSD2 was observed in renal cell carcinoma (RCC) and lung cancer.
- SUSD2 expression was frequently decreased in cancer tissues compared to normal adjacent tissues.
- Restoring SUSD2 inhibited proliferation and clonogenicity in RCC and lung cancer cells, while SUSD2 knockdown promoted A549 cell growth.
Conclusions:
- SUSD2 functions as a tumor suppressor gene (TSG) in both RCC and lung cancer.
- Downregulation of SUSD2 is associated with increased proliferation in these cancers.
- SUSD2 represents a potential therapeutic target for RCC and lung cancer treatment.
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