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Recent development of temperature-responsive surfaces and their application for cell sheet engineering.

Zhonglan Tang1, Teruo Okano1

  • 1Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.

Regenerative Biomaterials
|January 28, 2016
PubMed
Summary
This summary is machine-generated.

Cell sheet engineering offers a scaffold-free method for tissue regeneration. This technique harvests intact cell sheets using temperature changes, preserving cell integrity for transplantation and 3D tissue construction in regenerative medicine.

Keywords:
cell sheetcell sheet engineeringpoly(N-isoproplyacrylamide)temperature-responsive cell culture surface

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Area of Science:

  • Regenerative Medicine
  • Biotechnology
  • Tissue Engineering

Background:

  • Traditional tissue engineering often relies on biodegradable scaffolds.
  • Scaffold use can introduce limitations in cell viability and tissue integration.
  • Cell sheet engineering presents an alternative, scaffold-free approach.

Purpose of the Study:

  • To review recent advancements in cell sheet engineering.
  • To discuss the preparation of temperature-responsive surfaces.
  • To highlight the fabrication of 3D tissues and clinical applications.

Main Methods:

  • Culturing cells on temperature-responsive surfaces at 37°C.
  • Harvesting intact cell sheets by reducing temperature to 20°C.
  • Utilizing cell sheet layering and bioreactors for 3D tissue fabrication.

Main Results:

  • Cell sheets harvested without enzymatic treatment retain cell-cell junctions, surface proteins, and extracellular matrix.
  • Cell sheets are suitable for direct transplantation and building 3D constructs.
  • Integration of cell sheet technology with bioreactors enables complex tissue fabrication.

Conclusions:

  • Cell sheet engineering is a promising scaffold-free strategy for tissue regeneration.
  • The technique preserves native cell characteristics, enhancing therapeutic potential.
  • Ongoing clinical studies demonstrate the clinical applicability of cell sheet technology in regenerative medicine.