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Investigating the Role of Helicobacter pylori PriA Protein

Aparna Singh1, Dusan Blaskovic1, Jungsoo Joo1

  • 1National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.

Helicobacter
|January 29, 2016
PubMed

Insights

Helicobacter pylori PriA protein is essential for bacterial survival in mouse stomachs, aiding in DNA repair and resistance to stress. A priA mutant showed impaired colonization and increased sensitivity to DNA damage and acid.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Genetics

Background:

  • PriA protein, a DEXH-type DNA helicase, is crucial for bacterial replication restart and DNA repair.
  • PriA stabilizes stalled replication forks and is vital in organisms like E. coli and N. gonorrhoeae.
  • The priA gene was observed to be induced during Helicobacter pylori infection in mouse stomachs.

Purpose of the Study:

  • To investigate the role of Helicobacter pylori PriA protein in bacterial survival.
  • To determine PriA's function in mouse stomach colonization, intracellular survival, DNA repair, and stress resistance.

Main Methods:

  • Generating a priA null mutant strain of H. pylori.
  • Assessing bacterial colonization in mouse stomachs over time.
  • Evaluating survival in gastric epithelial and macrophage cells.
  • Testing sensitivity to DNA-damaging agents, acid, and oxidative stress.

Main Results:

  • The priA null mutant exhibited significantly reduced long-term colonization of mouse stomach mucosa compared to the wild-type.
  • PriA is important for the intracellular survival of H. pylori within epithelial and macrophage cells.
  • The priA mutant displayed increased sensitivity to DNA-damaging agents, acid, and oxidative stress.

Conclusions:

  • Helicobacter pylori PriA protein is essential for bacterial survival and persistence in the mouse stomach environment.
  • PriA contributes to H. pylori's ability to withstand host-induced stresses and maintain colonization.
Abstract

Keywords:
DNA helicaseHelicobacter pylorireplication fork stabilization

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