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Updated: Mar 26, 2026
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
Published on: July 11, 2020
Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera
Xiaolei Wang1, Songtao Gui1, Lei Pan2
1State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
Premise Of The Study:
Polymorphic microRNA (miRNA)-based microsatellite markers were developed to investigate the genetic diversity and population structure of Nelumbo nucifera (Nelumbonaceae).
Methods And Results:
A total of 485 miRNA-based microsatellites were found from the genomic DNA sequences of N. nucifera. After several rounds of screening, 21 primer pairs flanking di-, tri-, or pentanucleotide repeats were identified that revealed high levels of genetic diversity in four populations with two to five alleles per locus. The observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.803, respectively.
Conclusions:
The polymorphic microsatellite markers will be useful for studying the genetic diversity and population structure of N. nucifera.
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