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Related Experiment Video

Updated: Mar 26, 2026

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
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Seven major genomic deletions of vaccinia virus Tiantan strain are sufficient to decrease pathogenicity.

Yiquan Li1, Yuan Sheng1, Yunjie Chu2

  • 1Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun 130122, PR China; Jiang su Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.

Antiviral Research
|January 29, 2016
PubMed
Summary

A new attenuated vaccinia virus strain, TTVAC7, was created by deleting virulence genes. This safe viral vector maintains high immunogenicity while reducing toxicity and host range.

Keywords:
Attenuated vaccinia virus vectorImmunogenicityTiantan strainVirulence

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Area of Science:

  • Virology
  • Molecular Biology
  • Vaccine Development

Background:

  • Vaccinia virus Tiantan strain (VTT) is a basis for viral vector development.
  • Modifying VTT can enhance safety and efficacy for applications like vaccines.

Purpose of the Study:

  • To construct a multi-gene-deleted attenuated vaccinia virus strain, TTVAC7.
  • To evaluate TTVAC7's safety, immunogenicity, and potential as a viral vector or vaccine.

Main Methods:

  • Constructed shuttle vectors with enhanced green fluorescent protein (EGFP) as a selectable marker.
  • Utilized homologous recombination and Cre/LoxP system for precise gene knockout in VTT.
  • Performed in vivo and in vitro experiments to assess viral properties.

Main Results:

  • Successfully generated the attenuated strain TTVAC7 by deleting multiple non-essential genes.
  • Demonstrated a significantly narrowed host range and reduced toxicity of TTVAC7.
  • Confirmed that TTVAC7 retains high immunogenicity.

Conclusions:

  • TTVAC7 is a promising attenuated vaccinia virus with improved safety profile.
  • The developed strain holds potential for use as a safe viral vector or vaccine candidate.