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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Systematic approaches to identify functional lncRNAs.

Eric A Alcid¹, Toshio Tsukiyama²

¹Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA; Molecular and Cellular Biology Program, University of Washington and Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA.

Current Opinion in Genetics & Development

|January 29, 2016

Summary

Researchers propose new methods to identify the functions of long noncoding RNAs (lncRNAs). These methods focus on lncRNAs with dedicated preinitiation complexes (PICs) and those with highly regulated transcription, aiding in understanding their biological roles.

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Last Updated: Mar 26, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

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Published on: April 10, 2018

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Identification of Circular RNAs using RNA Sequencing

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Published on: November 14, 2019

Area of Science:

Genomics
Molecular Biology
RNA Biology

Background:

Long noncoding RNAs (lncRNAs) are transcribed across the genome.
While many lncRNAs are identified, their biological functions remain largely unknown.
Expression of some lncRNAs changes during cell differentiation and in disease.

Purpose of the Study:

To propose novel strategies for identifying functional long noncoding RNAs (lncRNAs).
To address the challenge of determining the biological roles of the vast majority of discovered lncRNAs.

Main Methods:

Identifying lncRNAs associated with dedicated preinitiation complexes (PICs).
Focusing on lncRNAs whose transcription levels are highly regulated.

Main Results:

The study proposes two distinct approaches to uncover lncRNA functions.
These methods aim to streamline the discovery of biologically active lncRNAs.

Conclusions:

Identifying lncRNAs with dedicated PICs offers a route to functional characterization.
Analyzing lncRNAs with regulated transcription is another key strategy for functional discovery.
These approaches are expected to advance the understanding of lncRNA roles in biological processes.