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Updated: Aug 17, 2026

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill
Published on: January 29, 2021
[Cryostability of yeast cells and their sensitivity to basic factors of freezing]
Abstract:
The cryostability of Saccharomyces cerevisiae cells was found to correlate with their sensitivity to some physico-chemical factors of freezing. The threshold of their resistance to the main factors of cryoconservation (a low-temperature shock, the presence of a salt, a change in the pH of the medium) was determined at a temperature value physiological for this microorganism. This work was done in order to substantiate and predict optimal freezing regimes while making microbial cells pass into the state of deep cryptobiosis.
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