Related Experiment Video

Updated: Mar 26, 2026

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

15.4K

Expression and functional role of miR-29b in renal cell carcinoma

Yi Xu1, Jianyong Zhu1, Zhangming Lei1

  • 1Department of Urology, Quzhou People's Hospital Quzhou 324000, Zhejiang, China.

International Journal of Clinical and Experimental Pathology
|January 30, 2016
PubMed
Abstract

Insights

MicroRNA-29b (miR-29b) is elevated in renal cell carcinoma (RCC), promoting tumor growth and invasion by suppressing KIF1B. This suggests miR-29b may serve as a prognostic marker for RCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are crucial regulators in various cancers, including renal cell carcinoma (RCC).
  • Understanding the specific roles of miRNAs like miR-29b in RCC pathogenesis is essential for developing targeted therapies.
  • The expression and function of miR-29b in RCC remain incompletely elucidated.

Purpose of the Study:

  • To investigate the expression profile of miR-29b in renal cell carcinoma (RCC).
  • To explore the functional significance of miR-29b in RCC cell proliferation, apoptosis, cell cycle, and invasion.
  • To identify and validate target genes of miR-29b involved in RCC pathogenesis.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) was used to determine miR-29b expression in ccRCC tissues and cell lines.
  • Lentivirus vectors were employed to manipulate miR-29b levels in SN12-PM6 cells to assess functional impacts.
  • Bioinformatics approaches were utilized to predict and investigate Kinesin Family Member 1B (KIF1B) as a potential miR-29b target.

Main Results:

  • miR-29b expression was significantly upregulated in both ccRCC tumor tissues and cell lines.
  • High miR-29b expression correlated with advanced Tumor-Node-Metastasis (TNM) stage and poorer overall survival in ccRCC patients.
  • Inhibition of miR-29b suppressed proliferation and invasion while promoting apoptosis in RCC cells, and directly downregulated KIF1B expression.

Conclusions:

  • miR-29b functions as an oncomiR in RCC, driving proliferation and invasion via suppression of its target gene, KIF1B.
  • miR-29b may serve as a valuable prognostic biomarker for renal cell carcinoma.
  • Targeting miR-29b could represent a potential therapeutic strategy for RCC.
Keywords:
Renal cell carcinomakinesin family member 1BmiR-29b

More Related Videos

miRNA Expression Analyses in Prostate Cancer Clinical Tissues11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.3K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.9K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K