High-Performance Affinity Chromatography: Applications in Drug-Protein Binding Studies and Personalized Medicine

Zhao Li1, Sandya R Beeram1, Cong Bi1

  • 1Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
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