Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondria01:37

Mitochondria

21.3K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
21.3K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

19.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
19.5K
Mitochondrial Membranes01:45

Mitochondrial Membranes

17.7K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
17.7K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

9.7K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alleviation of photoaging-associated MMP upregulation, prostanoid biosynthesis, and cell cycle arrest with titanium dioxide, zinc oxide, and inorganic-only (ZnO + TiO<sub>2</sub>) sunscreens.

Photochemistry and photobiology·2025
Same author

The Effect of Blue Light on Mitochondria in Human Dermal Fibroblasts and the Potential Aging Implications.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

Profiling the Anti-Photoaging Impact of Titanium Dioxide and Zinc Oxide Nanoparticles: A Focus on Signaling Pathways.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

Thoracic Cancer Exercise Services in Australia: A Point-Prevalence Survey.

Asia-Pacific journal of clinical oncology·2025
Same author

Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler.

SLAS technology·2024
Same author

Development and Application of Automated Sandwich ELISA for Quantitating Residual dsRNA in mRNA Vaccines.

Vaccines·2024
Same journal

An Integrated Skin Cell Atlas Decodes the Pilosebaceous Unit.

The Journal of investigative dermatology·2026
Same journal

Residual CSB activity explains mild UV-sensitive syndrome phenotype caused by CSB mutations.

The Journal of investigative dermatology·2026
Same journal

Charting a new melanoma risk factor: Satellite Naevus Prevalence in High-Risk and Population-Based Cohorts.

The Journal of investigative dermatology·2026
Same journal

Human keratinocytes and fibroblasts coordinate early cutaneous innate defenses against Candida auris.

The Journal of investigative dermatology·2026
Same journal

Merkel cells attenuate autoantigen-specific T cell driven skin inflammation in mice associated with reduced neutrophil recruitment.

The Journal of investigative dermatology·2026
Same journal

The response of human melanocytic nevi to simulated solar radiation assessed by single-nucleus RNA sequencing of frozen tissue.

The Journal of investigative dermatology·2026
See all related articles

Related Experiment Video

Updated: Mar 26, 2026

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
08:04

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

Published on: August 16, 2021

3.0K

Age-Dependent Decrease of Mitochondrial Complex II Activity in Human Skin Fibroblasts.

Amy Bowman1, Mark A Birch-Machin1

  • 1Dermatological Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.

The Journal of Investigative Dermatology
|February 2, 2016
PubMed
Summary
This summary is machine-generated.

Aging is linked to declining mitochondrial function, particularly Complex II activity in human skin fibroblasts. This decrease correlates with reduced gene and protein expression, especially in senescent cells.

More Related Videos

Author Spotlight: Unveiling Mitochondrial Function and Cellular Metabolic Adaptation in Metabolic Diseases
08:12

Author Spotlight: Unveiling Mitochondrial Function and Cellular Metabolic Adaptation in Metabolic Diseases

Published on: October 4, 2024

2.4K
Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
09:16

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

3.1K

Related Experiment Videos

Last Updated: Mar 26, 2026

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
08:04

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

Published on: August 16, 2021

3.0K
Author Spotlight: Unveiling Mitochondrial Function and Cellular Metabolic Adaptation in Metabolic Diseases
08:12

Author Spotlight: Unveiling Mitochondrial Function and Cellular Metabolic Adaptation in Metabolic Diseases

Published on: October 4, 2024

2.4K
Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
09:16

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

3.1K

Area of Science:

  • Gerontology
  • Mitochondrial Biology
  • Cellular Aging

Background:

  • The mitochondrial theory of aging posits that dysfunction in the electron transport chain leads to increased free radical generation.
  • Complex II of the electron transport chain is increasingly recognized for its role in aging, primarily from non-human studies.

Purpose of the Study:

  • To investigate the association between Complex II activity and aging in human skin.
  • To determine if Complex II dysfunction contributes to cellular senescence in aging skin.

Main Methods:

  • Assessed Complex II activity in fibroblasts and keratinocytes from human skin across a wide age range.
  • Measured transcript and protein levels of succinate dehydrogenase complex subunits A and B.
  • Compared Complex II activity in senescent versus non-senescent skin cells.

Main Results:

  • Complex II activity significantly decreased with age in fibroblasts but not keratinocytes.
  • A decline in Complex II subunit A and B transcript and protein levels was observed with aging.
  • Complex II activity specifically decreased in senescent skin cells.
  • Complex IV activity showed no age-related decline, suggesting specificity to Complex II.

Conclusions:

  • Aging is associated with reduced Complex II activity in human skin fibroblasts, linked to decreased gene and protein expression.
  • This decline is particularly evident in senescent skin cells, supporting Complex II's role in cellular aging.
  • The findings highlight Complex II as a potential key player in the mitochondrial theory of aging in human skin.