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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Affinity Chromatography01:03

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
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Affinity approaches in RNAi-based therapeutics purification.

Patrícia Pereira1, João A Queiroz1, Ana Figueiras2

  • 1CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|February 3, 2016
PubMed
Summary

RNA interference (RNAi) therapeutics utilize small and long non-coding RNAs (ncRNAs) for gene silencing. Affinity chromatography is emerging as a key method for purifying these ncRNAs for therapeutic applications.

Keywords:
Affinity chromatographyNon-coding RNAsRNA purificationReview

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Biotechnology

Background:

  • RNA interference (RNAi) mechanisms highlight RNA's critical biological roles.
  • RNAi-based technologies are advancing as potent tools for gene silencing and developing novel therapeutics for various human diseases.
  • Small and long non-coding RNAs (ncRNAs) are integral to RNAi and represent next-generation therapeutic agents.

Purpose of the Study:

  • To review the biogenesis pathways of regulatory ncRNAs.
  • To discuss recent advancements and applications of affinity chromatography in ncRNA purification.
  • To highlight the significance of affinity chromatography for high-purity ncRNA production.

Main Methods:

  • Review of literature on ncRNA biogenesis.
  • Analysis of recent developments in affinity chromatography techniques for RNA purification.
  • Discussion of the application of affinity chromatography in purifying small and long non-coding RNAs.

Main Results:

  • Affinity chromatography offers high specificity for ncRNA purification.
  • Innovative procedures are needed for efficient and high-purity RNA isolation.
  • Recent applications demonstrate the potential of affinity chromatography in this field.

Conclusions:

  • Affinity chromatography is crucial for purifying ncRNAs for therapeutic use.
  • Further development and application of affinity chromatography are expected to enhance RNA-based therapies.
  • The review provides insights into the future prospects of affinity chromatography in ncRNA purification.