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Published on: August 17, 2019
A calixpyridinium-based supramolecular tandem assay for alkaline phosphatase and its application to ATP hydrolysis
Kui Wang1, Jian-Hua Cui1, Si-Yang Xing1
1Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Key Laboratory of Inorganic-Organic Hybrid Functional Materials Chemistry (Tianjin Normal University), Ministry of Education, College of Chemistry, Tianjin Normal University, Tianjin 300387, P. R. China. hxxywk@mail.tjnu.edu.cn hxxyxsy@mail.tjnu.edu.cn.
Abstract:
We have successfully implemented the supramolecular tandem assay principle for the real-time, continuous, direct, and label-free monitoring of alkaline phosphatase activity through a fluorescence "switch-off" assay based on a novel calixpyridinium/dye reporter pair. Because several diseases can be preliminarily diagnosed in light of an abnormal level of alkaline phosphatase in serum, the application of tandem assays to selectively monitor alkaline phosphatase activity has feasible implications in disease diagnosis.
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