Related Experiment Video

Updated: Mar 26, 2026

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.5K

miR-708/LSD1 axis regulates the proliferation and invasion of breast cancer cells

Lin Ma1, Shan Ma2, Guimei Zhao3

  • 1Department of Neurology, Shanghai Tongji Hospital, Tongji University, School of Medicine, Shanghai, 200065, China.

Cancer Medicine
|February 3, 2016
PubMed

Insights

MicroRNAs (miRNAs) like miR-708 act as tumor suppressors in breast cancer by inhibiting cell growth and invasion. The miR-708/LSD1 pathway presents a potential therapeutic target for breast cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a leading cause of mortality in women globally.
  • MicroRNAs (miRNAs) are key regulators of cellular processes, including cancer development.
  • The specific role of miR-708 in breast cancer pathogenesis was previously undefined.

Purpose of the Study:

  • To investigate the function of miR-708 in breast cancer.
  • To identify the molecular targets of miR-708 in breast cancer cells.
  • To explore the potential of the miR-708/LSD1 axis as a therapeutic strategy.

Main Methods:

  • Forced expression and inhibition of miR-708 in breast cancer cell lines.
  • Luciferase reporter assays to confirm direct targeting of LSD1.
  • Analysis of cell proliferation and invasion assays.
  • Overexpression of LSD1 to assess its interaction with miR-708 effects.

Main Results:

  • Overexpression of miR-708 suppressed breast cancer cell proliferation and invasion.
  • Inhibition of miR-708 enhanced cell growth and invasion.
  • miR-708 directly targets the 3' untranslated region (3'UTR) of Lysine-Specific Demethylase 1 (LSD1).
  • LSD1 inhibition mimicked miR-708's tumor-suppressive effects, and LSD1 overexpression counteracted them.

Conclusions:

  • miR-708 functions as a tumor suppressor gene in breast cancer.
  • The miR-708/LSD1 signaling pathway is implicated in breast cancer development.
  • Targeting the miR-708/LSD1 axis offers a promising therapeutic avenue for breast cancer.
Keywords:
Breast cancerLSD1invasionmiR-708proliferation

More Related Videos

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

14.8K
Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence06:52

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Published on: November 5, 2021

3.8K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K