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[Features of determining autoantibodies to human myoglobin using an immunoenzyme analysis method]
Various procedures of immunoenzyme assay (ELISA) were studied for evaluation of autoantibodies against human myoglobin. Antigenic properties of myoglobin were lost after immobilization on solid-phase carrier, hence noncompetitive type of the assay is unsuitable for these analyses. Exogenous myoglobin did not inhibit practically the blood serum immunoglobulins, which have reacted with immobilized myoglobin. These immunoglobulins appear to be autoantibodies against other antigens cross reacting with myoglobin. The procedure of competitive ELISA, involving solid phase immobilized specific antimyoglobin antibodies, was developed and assayed; an obligatory condition for the procedure developed is separation of immunoglobulins from myoglobin. Myoglobin and its naturally occurring antibodies were found as immunocomplexes in circulation.
Various procedures of immunoenzyme assay (ELISA) were studied for evaluation of autoantibodies against human myoglobin. Antigenic properties of myoglobin were lost after immobilization on solid-phase carrier, hence noncompetitive type of the assay is unsuitable for these analyses. Exogenous myoglobin did not inhibit practically the blood serum immunoglobulins, which have reacted with immobilized myoglobin. These immunoglobulins appear to be autoantibodies against other antigens cross reacting with myoglobin. The procedure of competitive ELISA, involving solid phase immobilized specific antimyoglobin antibodies, was developed and assayed; an obligatory condition for the procedure developed is separation of immunoglobulins from myoglobin. Myoglobin and its naturally occurring antibodies were found as immunocomplexes in circulation.