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MinION Analysis and Reference Consortium: Phase 1 data release and analysis.

Camilla L C Ip¹, Matthew Loose², John R Tyson³

¹Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.

|February 3, 2016

Summary

This summary is machine-generated.

The MinION Analysis and Reference Consortium (MARC) evaluated the reproducibility of the new MinION DNA sequencing device across multiple labs. Phase 1 data show the device

Keywords:

MinION NanoOK data release long reads marginAlign minoTour nanopore sequencing third-generation sequencing

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Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

The MinION is a novel, portable DNA sequencing device.
The MinION Access Programme (MAP) provided early access to this technology.
The MinION Analysis and Reference Consortium (MARC) was formed to standardize its use.

Purpose of the Study:

To evaluate the reproducibility of MinION sequencing performance across multiple independent laboratories.
To provide standardized protocols and reference data for the MinION platform.
To analyze the characteristics of MinION sequencing runs, including data quality and consistency.

Main Methods:

Five laboratories on two continents participated in Phase 1 of the study.
A standardized protocol, revised from Oxford Nanopore Technologies (ONT), was used.
DNA samples from a control strain of Escherichia coli K-12 were prepared and sequenced.

Main Results:

Preliminary analysis of Phase 1 data provides insights into typical run characteristics.
Data cover consistency, rate, volume, and quality of sequencing output.
The study details the specific protocol employed for reproducibility assessment.

Conclusions:

Phase 1 established a baseline for MinION performance reproducibility.
Further analysis and Phase 2 experiments are ongoing to enhance MinION capabilities.
The MARC initiative aims to foster community-driven improvements in nanopore sequencing technology.